Pd12-12 Assessing the Influence of Rem Sleep on Bladder Function: Insights From a Rat Model of Sleep Disruption

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology (PD12)1 May 2024PD12-12 ASSESSING THE INFLUENCE OF REM SLEEP ON BLADDER FUNCTION: INSIGHTS FROM A RAT MODEL OF SLEEP DISRUPTION Shada S. Sinclair, Bernardita M. Ljubetic, Claire S. Burton, Ashu Mohammad, Craig V. Comiter, and Amy D. Dobberfuhl Shada S. SinclairShada S. Sinclair , Bernardita M. LjubeticBernardita M. Ljubetic , Claire S. BurtonClaire S. Burton , Ashu MohammadAshu Mohammad , Craig V. ComiterCraig V. Comiter , and Amy D. DobberfuhlAmy D. Dobberfuhl View All Author Informationhttps://doi.org/10.1097/01.JU.0001008772.30001.48.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Circadian disruption has been implicated as a cause of storage and voiding dysfunction. Oscillatory genes throughout the body are regulated centrally by the suprachiasmatic nucleus via the day-night light cycle. We aimed to assess the impact of sleep interruption on circadian bladder function. METHODS: Adult female rats (n=18) were divided into 3 groups [modified multiple platform method (MMPM), wire screen control (WS), home cage control (HC)]. MMPM and WS animals were placed in a plastic chamber (90×50 cm) with platforms (6 cm diameter) surrounded by 2 cm water 8 hours per day for 21 days during the lights on rest phase. MMPM disrupted rapid eye movement (REM) sleep, when REM atonia caused rats to fall into water. WS animals were prevented from contacting water, to simulate the novel environment, accounting for non-sleep disruption stress. HC controls were kept in their home cages. Ambulatory bladder function was assessed weekly using the 2-hour filter paper test. Animals were euthanized in two time phases (7 AM, 7 PM). Bladders and hippocampi were collected for bulk mRNA sequencing. RESULTS: There was no animal mortality. No animal was excluded from analysis. Animals were matched at baseline, with no difference in mean volume per void for MMPM (0.73±0.32 mL): versus HC (0.62±0.32 mL, p=0.555); versus WS (0.54±0.20 mL; p=0.235). On day 21 paired t-test within group analysis compared to baseline, MMPM animals demonstrated an increase in urinary frequency (#voids 3.00±1.26 versus 22.8±20.0, p=0.061) and significant reduction in volume per void (0.73±0.32 versus 0.15±0.13 mL, p=0.014). HC demonstrated a non-significant increase in #voids (p=0.136) and non-significant reduction in volume per void (p=0.060). WS did not significantly increase #voids (p=0.728) or volume per void (p=0.579). On mRNA differential expression (DE) analysis for MMPM versus HC, hippocampus (2757 DE genes) was more affected by lack of REM sleep than bladder (1404 DE genes). A similar number of DE genes were noted in bladder tissue for AM (979 DE genes) and PM (965 DE genes) euthanasia (Figure 1). CONCLUSIONS: Our findings suggest a link between interruption of REM sleep, increase in urinary frequency and decrease in volume per void. Future research may provide insights into targeted interventions for patients with circadian bladder dysfunction. Download PPT Source of Funding: The McCormick and Gabilan Faculty Fellowship Award © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e261 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Shada S. Sinclair More articles by this author Bernardita M. Ljubetic More articles by this author Claire S. Burton More articles by this author Ashu Mohammad More articles by this author Craig V. Comiter More articles by this author Amy D. Dobberfuhl More articles by this author Expand All Advertisement PDF downloadLoading ...