Pd53-04 Phosphatidylserine Positive (Ps ⁺ ) Live Sperm: An Indicator of Fertility in Patients With Varicocele

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You have accessJournal of UrologyInfertility: Basic Research & Pathophysiology (PD53)1 May 2024PD53-04 PHOSPHATIDYLSERINE POSITIVE (PS+) LIVE SPERM: AN INDICATOR OF FERTILITY IN PATIENTS WITH VARICOCELE David Charles, Christina George, Bindica Poudel, Laura Church, Yazan Hamzeh, Kayla Boyle, Claire Cielencki, Ryan Smith, Parviz Kavoussi, David Manka, and Jeffrey Lysiak David CharlesDavid Charles , Christina GeorgeChristina George , Bindica PoudelBindica Poudel , Laura ChurchLaura Church , Yazan HamzehYazan Hamzeh , Kayla BoyleKayla Boyle , Claire CielenckiClaire Cielencki , Ryan SmithRyan Smith , Parviz KavoussiParviz Kavoussi , David MankaDavid Manka , and Jeffrey LysiakJeffrey Lysiak View All Author Informationhttps://doi.org/10.1097/01.JU.0001009456.01770.91.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: We recently found that phosphatidylserine (PS) is exposed on live sperm, is necessary for sperm: egg fusion and may be a biomarker for fertilization competent sperm. Varicocele is associated with a decrease in sperm quality and an established etiology of male infertility; yet, some men with varicoceles are able to father children. In this study we analyzed semen samples from pregnancy proven donors with normal semen parameters and from infertile men seeking varicocele treatment for the percentage of PS positive live (PS+live) sperm. The aim was to determine if the percentage of PS+live sperm (PS Score) differs in the two groups. METHODS: Semen samples were collected from men with various degrees of varicocele and analyzed with semen analysis. Cryobank frozen semen samples from pregnancy proven men with normal semen parameters were purchased from two cryobanks. Screening for PS+live sperm was performed on all samples by incubating samples with fluorescently tagged annexin (PS+) and 7AAD (dead) and subsequently analyzed by flow cytometry to determine the percentage of PS+live cells. RESULTS: Semen samples from pregnancy proven donors with normal semen parameters (n=25) had a mean PS Score (%PS+live sperm) of 82% (sem±1.44) in comparison to the PS Score in samples from men with varicoceles (n=19) which varied greatly and had a significantly lower %PS+live sperm mean of 63% (sem±4.99) (p<0.05; see Figure). Three patients with varicoceles reported pregnancies without undergoing repair after enrolling in the study. They had PS Scores of 88%, 70%, and 78% respectively. CONCLUSIONS: These results suggest that the percentage of PS+live sperm (PS Score) in an ejaculate may be an indicator of fertility in patients with varicoceles. Patients demonstrating a PS Score above 63% are more likely to spontaneously conceive than patients with a lower PS Score. In fact, 33% (3/9) of patients with varicoceles that had a PS Score above 63% achieved a pregnancy spontaneously compared to 0% below a PS Score of 63%. Download PPT Source of Funding: Commonwealth Commercialization Fund and Private funding © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1139 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information David Charles More articles by this author Christina George More articles by this author Bindica Poudel More articles by this author Laura Church More articles by this author Yazan Hamzeh More articles by this author Kayla Boyle More articles by this author Claire Cielencki More articles by this author Ryan Smith More articles by this author Parviz Kavoussi More articles by this author David Manka More articles by this author Jeffrey Lysiak More articles by this author Expand All Advertisement PDF downloadLoading ...