Pd41-04 Density of Cd163+ Tumor-Associated Macrophage Infiltration Predicts Prognosis of Upper Tract Urothelial Carcinoma Patients After Radical Nephroureterectomy

You have accessJournal of UrologyBladder Cancer: Upper Tract Transitional Cell Carcinoma III (PD41)1 May 2024PD41-04 DENSITY OF CD163+ TUMOR-ASSOCIATED MACROPHAGE INFILTRATION PREDICTS PROGNOSIS OF UPPER TRACT UROTHELIAL CARCINOMA PATIENTS AFTER RADICAL NEPHROURETERECTOMY Shang Yuan Hsu, Chien-Hui Ou, and Hau Chern Jan Shang Yuan HsuShang Yuan Hsu , Chien-Hui OuChien-Hui Ou , and Hau Chern JanHau Chern Jan View All Author Informationhttps://doi.org/10.1097/01.JU.0001008568.76803.f1.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To investigate the influence of CD163+ tumor-associated macrophage (TAM) infiltration density on oncological outcomes among upper tract urothelial carcinoma (UTUC) patients. METHODS: Immunohistochemical (IHC) analyses were performed on paraffin-embedded human tissue sections from blocks of UTUC tumors (N=136) using a monoclonal antibodies anti-CD163 for M2-phenotype macrophage. Next, CD 163+ TAMs from IHC staining can be scored from three hotspots (areas with the most intra-tumoral macrophages) intratumorally at a magnification of 400×. The mean of the density (count/mm2) obtained in three different areas was used for the data analysis. According to the percentile ranking of CD163+ TAM density, we classified patients into high, intermediate, and low CD163+ TAMs groups. Kaplan-Meier analysis was conducted to evaluate the effect of CD163+ TAMs density on metastasis-free survival (MFS) and cancer-specific survival (CSS) in UTUC after RNU. RESULTS: The samples were divided into low-level (N=46), intermediate-level (N=52) and high-level infiltration groups (n=38) by percentile ranking of CD163+ TAMs density. The MFS and CSS of UTUC patients in the high-level CD163+ TAMs density group were significantly shorter than those in the intermediate-and low-level CD163+ TAM density group (p<0.001). Multivariate Cox regression analysis demonstrated CD163+ TAMs density was an independent risk factor for a poorer MFS and CSS (HR of MFS=3.771, 95% CI 1.515–5.385, p=0.008; HR of CSS=2.402, 95% CI 1.345–4.287, p=0.003). CONCLUSIONS: The infiltration of CD163+ TAMs in UTUC tissue is an independent risk factor for the prognosis of UTUC patients. High-level infiltration of CD163+ TAMs is associated with shorter MFS and CSS. Download PPT Source of Funding: National Health Insurance © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e887 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Shang Yuan Hsu More articles by this author Chien-Hui Ou More articles by this author Hau Chern Jan More articles by this author Expand All Advertisement PDF downloadLoading ...