The RNA-programmed CRISPR-Cas12a activity requires two factors: complementarity between the guide RNA and the dsDNA, and a short Palindromic Adjacent Motif (PAM) flanking the target. In their Research Article (e202319677), Alessandro Porchetta et al. rationally designed PAM-engineered DNA hairpins as input-responsive activators of Cas12a. By utilizing the trans-cleavage activity of Cas12a as a signal transduction method, the authors demonstrated the rapid, one-pot detection of IgG antibodies and small molecules.
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Bagheri et al. (2024) studied this question.
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