Pd25-10 Intravesical Instillation of Epidermal Growth Factor Could Lessen the Inflammation and Ameliorate the Oxidative Stress in Rats With LPS-Induced Interstitial Cystitis

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Interstitial Cystitis (PD25)1 May 2024PD25-10 INTRAVESICAL INSTILLATION OF EPIDERMAL GROWTH FACTOR COULD LESSEN THE INFLAMMATION AND AMELIORATE THE OXIDATIVE STRESS IN RATS WITH LPS-INDUCED INTERSTITIAL CYSTITIS Che-Hsueh Yang, Yen-Chuan Ou, Min-Che Tung, and Chi-Chien Lin Che-Hsueh YangChe-Hsueh Yang , Yen-Chuan OuYen-Chuan Ou , Min-Che TungMin-Che Tung , and Chi-Chien LinChi-Chien Lin View All Author Informationhttps://doi.org/10.1097/01.JU.0001008584.88541.ff.10AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Epidermal growth factor (EGF) is polypeptides consisting of 53 amino acid residues, and it can help promote cells' growth and prevent oxidative injuries. In the recent five years, the intravesical instillation of EGF in interstitial cystitis (IC) was barely in progress. This study was the first one discussing the efficacy of intravesical instillation of EGF in rats with LPS-induced IC. METHODS: There were 15 female Sprague-Dawley rats used in this experiment, and they were divided into sham control, IC, and treatment group. The commercialized EGF product NewEpi (GoodCare Biotech, Taipei, Taiwan) was utilized. We employed 150 μl LPS to induce IC in rats, and then each rat in treatment group was infused with 400 սl NewEpi solution on day 0, day 7, and day 14 after IC induction. On day 21, the urinary bladder (UB) tissues were retrieved to analyze the inflammatory cytokines and oxidative markers. Tukey's HSD test was adopted to determine the differences among multiple treatments in one-way ANOVA. RESULTS: After instilling EGF, the urothelial inflammation was lessened, the fibrosis was ameliorated, the mast cells' infiltration was improved, and the urothelial denudation was recovered (Figure 1). In our results, EGF could remarkably down-regulate the pro-inflammatory and pro-fibrotic cytokines such as TNF-α, IL-6, and IL-1β (Figure 2A). In oxidative stress assessment, after instilling EGF, the free radicals' scavengers, GSH and SOD, were all significantly up-regulated, and the lipid peroxidation product MDA was lowered in UB (Figure 2B). CONCLUSIONS: By instilling EGF in rats with LPS-induced IC, urothelial inflammation and fibrosis were lessened. The mechanisms were postulated that it could down-regulate inflammation-related cytokines and up-regulate free radicals' scavengers. EGF could effectively lower the peroxidation of UB, and it is worth being studied in human clinical trial. Download PPTDownload PPT Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e540 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Che-Hsueh Yang More articles by this author Yen-Chuan Ou More articles by this author Min-Che Tung More articles by this author Chi-Chien Lin More articles by this author Expand All Advertisement PDF downloadLoading ...