Mp43-19 Novel Nanoparticle Coatings Demonstrate Antibacterial and Antibiofilm Properties Against Multiple Uropathogens

You have accessJournal of UrologySurgical Technology & Simulation: Instrumentation & Technology II (MP43)1 May 2024MP43-19 NOVEL NANOPARTICLE COATINGS DEMONSTRATE ANTIBACTERIAL AND ANTIBIOFILM PROPERTIES AGAINST MULTIPLE UROPATHOGENS Juan Sebastian Rodriguez-Alvarez, Yue Xu, Jorge Gutierrez-Aceves, Smita De, Aaron Miller, and Vijay Krishna Juan Sebastian Rodriguez-AlvarezJuan Sebastian Rodriguez-Alvarez , Yue XuYue Xu , Jorge Gutierrez-AcevesJorge Gutierrez-Aceves , Smita DeSmita De , Aaron MillerAaron Miller , and Vijay KrishnaVijay Krishna View All Author Informationhttps://doi.org/10.1097/01.JU.0001008720.96896.83.19AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Device-associated infections (DAI) are often associated with biofilm formation and ultimately require device removal. Silver (Ag) coatings have been used to minimize infections due to their intrinsic antibacterial properties, although gold-silver mixtures have demonstrated superior antimicrobial activity. Polyhydroxy-fullerene (PHF) Ag nanoparticles (NP) have shown increased antibacterial activity over commercial Ag NPs. We designed novel antimicrobial NPs composed of gold and Ag with PHF (AuAgNPs), hypothesized to enhance reactive oxygen species development. Coatings of AuAgNPs were evaluated for antimicrobial and anti-biofilm formation properties against several pathogens associated with urological DAI. METHODS: AuAgNPs were synthesized with PHF and then dry-coated on polyurethane discs. To induce biofilm formation on surfaces, coated and uncoated discs were incubated overnight in broth containing uropathogens. Bacterial strains included Escherichia coli, Enterococcus faecalis, Klebsiella oxytoca, Enterobacter hormaechei, Staphylococcus aureus and Staphylococcus epidermidis isolated from infected penile prostheses or ureteral stents. The surrounding broth was sampled and serially diluted. Biofilms were detached from the discs into phosphate-buffered saline, then serially diluted. All dilutions were plated and incubated to quantify colony-forming units (CFU) and CFU/mL, which was used to calculate reductions in biofilm and free-floating (planktonic) bacteria. All experiments were performed in triplicate. RESULTS: We observed significant antibacterial and antibiofilm activity of AuAgNPs against all tested bacterial species, ranging from 0.71 to 6.2 average logarithmic CFU/mL reduction. Notably, S. aureus biofilm and S. epidermidis free-floating bacteria were among the largest reductions observed (Figure 1). CONCLUSIONS: AuAgNP coatings demonstrate significant antibacterial and antibiofilm properties against a range of DAI bacterial strains. These findings suggest that AuAgNPs have the potential to reduce implant colonization and associated infections across various medical devices. Download PPT Source of Funding: CCF Catalyst grant, RPC © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e701 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Juan Sebastian Rodriguez-Alvarez More articles by this author Yue Xu More articles by this author Jorge Gutierrez-Aceves More articles by this author Smita De More articles by this author Aaron Miller More articles by this author Vijay Krishna More articles by this author Expand All Advertisement PDF downloadLoading ...