In this protocol, an easy and cost-friendly method to form mesenchymal stromal cell (MSC) spheroids was specified. The MSC spheroids would form in 1-3 days and are suitable for endothelial differentiation. Accutase treatment can be used to disassociate the spheroids into single cells, for further analysis. Multiple growth factors were used to differentiate MSC Spheroids into endothelial-like spheroids, which express endothelial markers such as von willebrand factors (vWF) and CD31 detected by immunofluorescence.
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Simeng Li (2024) studied this question.
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