Study on the Detection Technology of Injection Residue in the Trunk of Large (Ancient) Pine

The injection-dry application technique is a proactive technique to prevent Pine wilt disease. The study selected Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan six districts and counties (units) of the big (ancient) Pine (Pinus massoniana) as the research object, the use of injection of dry application method to clarify the prevention of Pine wilt disease by 2.3% Emamectin Benzoate and 5% abamectin, the use of high performance liquid chromatography determination of the injection of dry application of 180d and High performance liquid chromatography (HPLC) was used to determine the residue of Emamectin Benzoate in Pinus sylvestris after 180d and 360d of dry application. The results showed that Emamectin Benzoate and abamectin injection dry application of six districts and counties (units) of Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan, large (ancient) Pine wilt disease prevention and control effect is significant, the prevention effect of the consecutive 2a have reached more than 99%; In addition, the establishment of the pony pine in the Emamectin Benzoate and abamectin residue detection method, the side branch in the Emamectin Benzoate and abamectin In addition, a method was established to detect the residues of Emamectin Benzoate and abamectin in Pinus sylvestris, and the recoveries of Emamectin Benzoate and abamectin in the lateral branches exceeded 80%, and the analytical methods of Emamectin Benzoate and abamectin met the requirements of residue detection; the highest residue concentrations of Emamectin Benzoate and abamectin in the lateral branches of Pinus sylvestris were 2.93±1.63mg kg⁻¹ and 2.93±0.69mg kg⁻¹, respectively, 180d after injection drying. In the second year, the residue concentrations decreased, and the highest concentrations were more than 1.93±1.4 mg kg⁻¹ and 0.71±0.49 mg kg⁻¹ in the lateral branches of pine trees injected with mevinphos and abamectin, respectively.