Authentication of SSR Molecular Markers Associated With Genetic Male Sterility in Cotton (Gossypium Hirsutum L.) Parental Lines

Genetic male sterility serves as a highly efficient approach for generating hybrid cotton.In India, successful hybrid cotton development has predominantly relied on the utilization of lines containing the ms 5 ms 6 gene among various identified GMS gene-containing lines.In this current research, the validation of three GMS female lines, along with their fertile counterparts and the original non-GMS parental lines, was conducted using SSR markers with the primers BNL 1227 and NAU 2176.At the Main Cotton Research Station, Athwa Farm, N.A.U, Surat, India, three GMS female genotypes, their corresponding fertile counterparts, and the original parental lines lacking GMS were employed for the validation of molecular markers associated with GMS in cotton.This validation process involved DNA extraction and subsequent PCR amplification.Our investigations yielded conclusive evidence regarding the presence of the sterility gene in GMS lines.This demonstrates the genetic purity of the genotypes, and it was observed that the selected primers effectively validated both GMS and their respective original parental groups.Consequently, the primers BNL 1227 and NAU 2176 provide a rapid and dependable method for identifying the genetic male sterility system controlled by the ms5ms6 gene in cotton (G.hirsutum L.).Moreover, they serve as an efficient alternative to the time-consuming and labor-intensive grow-out test (GOT) for cotton.