FIGURE 6 from Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent “NoGlow” Mice

NoGlow mice reveal unappreciated metastatic dynamics independent of the primary tumor. A, Tumor growth of triple transgenic (3 ×) B16-F10 cells (10⁵) after subcutaneous implantation into the flank of WT C57Bl/6 (n = 2), CAG-driven Full-body WT GFP/Luc expressing mice (n = 3), CMV cre littermates positive (n = 8) or negative (n = 5) for the NoGlow construct, or SCID beige (n = 5) mice. B, Bioluminescence imaging of lungs at the time of euthanasia for each mouse from A. Robust signal is detected only in NoGlow+ lungs. *Indicates no detectable primary tumor at the end of experiment. C, Bioluminescence intensity of individual lungs from B. NoGlow+ lungs contained significantly more positive Luc signal than SCID, WT, or NoGlow− animals. D, Plot of tumor volume at time of euthanasia (x) by total lung radiance (y) for individual animals from A. P values were determined by one-way ANOVA with Tukey correction. All P values represent mean ± SEM.