FIGURE 6 from Low Exposures to Amphibole or Serpentine Asbestos in Germline Bap1-mutant Mice Induce Mesothelioma Characterized by an Immunosuppressive Tumor Microenvironment

Expression of IL6 and IL10 in ascites and Bap1 and p16Ink4a in MM tumors from chrysotile-exposed Bap1+/⁻ mice. A, Concentration of IL6 in ascites of Bap1+/⁻ mice compared with that from Bap1+/+ mice with MMs induced by chrysotile. Ascitic fluids were obtained from 5 Bap+/+ mice and 4 Bap1+/⁻ mice with chrysotile-induced MMs. B, Expression of IL10 in ascites of 4 Bap1+/⁻ mice versus that in ascites of 5 Bap1+/+ mice with MMs induced by chrysotile. C, Immunoblot analysis of tumor suppressors Bap1, Nf2, and p16Ink4a in MM tumors from 1 chrysotile-exposed Bap1+/+ mouse and 6 chrysotile-exposed Bap1+/− mice. NMC, normal (mouse) mesothelial cells; Vinculin, loading control.