A step-emulsion based digital-RPA for Pathogenic bacteria detection

Foodborne bacteria are major causes that affect human health. Development of new method that could achieve Rapid, sensitive and quantitative detection of pathogen is urgently needed. In this research, a step emulsion microfluidic,combined with droplet-based digital-RPA, was developed to detect Vibrio parahaemolyticus , a major seafood-borne pathogenic bacteria. Specific and rapid detection of Vibrio parahaemolyticus in 30 min has been achieved by this new device, with a detection limit of 10 CFU/μL, about 10-times lower than classical tube-based RPA. This device was demonstrated to be a promising tool for detection of pathogenic bacteria.