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Abstract Methylmercury (MeHg), an environmental pollutant, is a ubiquitous neurotoxicant that ultimately leads to neurodegenerative diseases. New experimental models are needed for public health toxicity testing associated with this pollutant. In this study, a human pluripotent stem cell‐based brain‐on‐a‐chip, named Communicative Astrocyte–Neuron DYnamics chip (CANDY chip), is reported, which enables observation of the interactions of neurons with astrocytes and accurate assessment of responses to penetration of MeHg across the blood‐brain barrier. Five neurophysiological characteristics of the CANDY chip provide an exact emulation of the brain: i) co‐culture of neurons with astrocytes, ii) transport of nutrients from blood vessels to neurons via astrocytes, iii) glia‐to‐neurons ratio of 1:1, iv) low‐speed flow of brain interstitial fluid, and v) neuroprotection by astrocytes against MeHg‐induced toxicity. The remarkable vulnerability of neurons is identified to MeHg toxicity as shattered neurites and verifies the tolerance of astrocytes to MeHg toxicity despite morphological changes such as cell shrinkage. It is also confirmed that the chip faithfully recapitulates actual neurophysiological responses through high neuronal cell viability because of the astrocytes and increased brain‐derived neurotrophic factor as a neuroprotector. The CANDY chip can be a powerful platform for accurate neurotoxicity testing and modeling of complex neurodegenerative diseases.
Choi et al. (Fri,) studied this question.