Initial Evaluation of the Efficiency of Cryoprotectant-Free Vitrification in PCR Tubes on a Low Number of Human Spermatozoa

Objectives: To inititally evaluate the efficiency of cryoprotectant-free vitrification in PCR tubes on a low number of human spermatozoa. Methods: A laboratory experimental study was conducted on 30 severe oligospermia treated at the Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University. The semen samples were vitrified and thawed at the following times, then sperm concentration, morphology, viability, and motility were evaluated after thawing. Results: The viable CSF, motile CSF when vitrifing without using CPAs were 50.45 ± 4.63; 43.35 ± 4.81 (1-week storage), 45.24 ± 4.33; 37.24 ± 4.39 (4-week storage) and 40.69 ± 3.96; 33.96 ± 4.14 (8-week storage), respectively. Conclusion: Vitrification of human sperm can be achieved without cryoprotectants and could be recommended for routine assisted reproductive technology.