FIGURE 4 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate

Expression of VCAN in tissues and cells. A, VCAN protein expression from IHC compared with gene expression from RNAscope. Spearman rank correlation. B, Comparison of FAP⁺ cells and percentage of α-SMA staining with number of VCAN⁺ cells. Each dot is colored to represent TIP. Red = inflamed, Gray = excluded, Blue = desert. C, Structure of VCAN isoforms and the number of predicted CS chains attached. qRT-PCR for VCAN isoforms completed on RNA extracted from frozen TNBC tissues (N = 9; D), fibroblasts (E), primary fibroblasts from healthy patients (N = 2) and from paired tumor and surround areas of patients with TNBC (N = 4), immortalized mammary fibroblast cell line (HMF3S; N = 4; TNBC cell lines HCC38 and MDAMB468 (N = 4; F). Coculture of HMF3S with HCC38 and MDAMB468 at a 4:1 ratio (N = 4; G). CT values for each gene normalized to CT values for housekeeping gene RPS13 to form ΔCT. **, P < 0.01.