FIGURE 2 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate

VCAN is associated with T-cell localization. A, Matrix index from Pearce and colleagues (14). Arrows indicate upregulation or downregulation at gene (G) or protein (P) level. B, Level of gene expression of top five upregulated molecules in 100 samples from TCGA dataset. C, scRNA-seq expression of the five genes indicating which cell types express the genes and the percentage of those cells that are positive. Data obtained from Wu and colleagues (25). D, IHC completed on consecutive TMA cores for COL11A1, COMP, CTSB, FN1, and VCAN. Spearman rank correlation of the expression of proteins from IHC analysis of eight TMA cores. **, P < 0.01 (N = 8). E, Comparison of dual RNAscope for CTSB (green) and VCAN (red) with IHC staining for CD8.