FIGURE 6 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate

CS isomers expression in tissues. A, Structure of CS isoforms following digestion of the chains with ChABC. CS extracted from FFPE tissues in the epithelial zone and stromal zone. CS isoforms analyzed by MS to identify proportion of each isomer. B, Comparison of isoforms in excluded and inflamed tissues within the different zones. Two-way ANOVA. C, Comparison of isomer in the different zones within excluded and inflamed tissues. Two-way ANOVA. D, CS-A/C ratio determined for the different regions of each phenotype and compared. ANOVA. E, Level of sulfation per CS disaccharide in the different regions of each phenotype compared. *, P < 0.05; **, P < 0.01; ****, P < 0.001.