FIGURE 7 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate

Effect of CS toward T-cell invasion. A, CS MS completed on the crude and enriched protein from HMF3S and HCC38 cell lines. CS isomers and A/C ratio determined. B, Transwell assay completed with 5 µg of each protein sample. Each sample was treated with ChABC for comparison. Heat map shows level of T cells invaded over time for one T-cell donor. Graphs show the level of migrated cells for HMF3S (C) and HCC38 (D) with error bars showing replicates with different T-cell donors.