Methylmercury-Induced Ferroptosis May Be Attenuated by Vitamin K in PC12 Cells

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Methylmercury-Induced Ferroptosis May Be Attenuated by Vitamin K in PC12 Cells † by Yanli LinYanli Lin SciProfiles Scilit Preprints.org Google Scholar Department of Preventive Medicine and Health Laboratory, School of Medicine, Jiangsu University, Zhenjiang 212013, China † Presented at the 1st International Electronic Conference on Toxics, 20–22 March 2024; Available online: https://sciforum.net/event/IECTO2024. Proceedings 2024, 102(1), 37; https://doi.org/10.3390/proceedings2024102037 Published: 3 April 2024 Download keyboard_arrow_down Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: methylmercury; vitamin K; ferroptosis; FSP1 Introduction: As a ubiquitous environmental pollutant, methylmercury (MeHg) induces toxic effects in the nervous system. However, the exact mechanism of its neurotoxicity has not been fully elucidated. Ferroptosis may be related to methylmercury toxicity and methylmercury-induced ferroptosis may be attenuated by vitamin K.Methods: PC12 cells with neuron-like characteristics were selected and treated with different concentrations of MeHg (0, 1, 2.5, 5, and 10 μM) for 6 h. CCK8 was used to detect cell viability; a FerroOrange fluorescent probe was used to detect the level of free ferrous ions in cells; the microplate method was used to detect the level of reduced GSH in cells; FSP1, SLC7A11, and GPX4 protein expressions were detected by Western blotting; and the changes in lipid ROS content in cells were detected by flow cytometry. In the vitamin K intervention experiment, the MeHg group was treated with 5 μM MeHg for 6 h, the vitamin K + MeHg group was pretreated with vitamin K (0, 10, 20, 40, 80, and 100 μM) for 1 h and then co-treated with 5 μM MeHg for 6 h, and the changes in intracellular lipid ROS content were detected by flow cytometry.Results: MeHg decreased the viability of PC12 cells in a dose-dependent manner. Meanwhile, the level of free ferrous ions in cells was significantly increased; the content of lipid ROS in cells was also significantly increased; the expressions of FSP1, SLC7A11, and GPX4 decreased; and the level of GSH in cells was significantly reduced. After vitamin K intervention, cell viability increased in a dose-dependent manner compared to the MeHg group, and intracellular lipid ROS content was significantly reduced after treatment with 80 μM vitamin K.Conclusions: MeHg can induce ferroptosis in neuron-like cells, and vitamin K intervention can alleviate MeHg-induced cytotoxicity and ferroptosis; thus, its exact mechanism is worthy of further investigation. FundingThis work was supported by the Natural Science Foundation of China (No. 30872139, No. 81273124).Institutional Review Board StatementNot applicable.Informed Consent StatementNot applicable.Data Availability StatementData will be made available on request.Conflicts of InterestThe author declares no conflict of interest.Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Lin, Y. Methylmercury-Induced Ferroptosis May Be Attenuated by Vitamin K in PC12 Cells. Proceedings 2024, 102, 37. https://doi.org/10.3390/proceedings2024102037 AMA Style Lin Y. Methylmercury-Induced Ferroptosis May Be Attenuated by Vitamin K in PC12 Cells. Proceedings. 2024; 102(1):37. https://doi.org/10.3390/proceedings2024102037 Chicago/Turabian Style Lin, Yanli. 2024. "Methylmercury-Induced Ferroptosis May Be Attenuated by Vitamin K in PC12 Cells" Proceedings 102, no. 1: 37. https://doi.org/10.3390/proceedings2024102037 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.