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This project, grounded in Biomaterials and Bioinstrumentation courses within Biomedical Engineering, aims to enhance the optical transparency and resolution of microfluidic devices fabricated using low-cost digital light processing (DLP) 3D printing.Prioritizing affordability and accessibility, we modified a commercially available 3D printer (Phrozen Sonic Mini 8K) by substituting the build plate with surface-treated glass slides.These slides underwent surface treatment to effectively modify their hydroxy-group-rich surface with a hydrophobic agent (3-(Trimethoxysilyl)propyl methacrylate (TSMPMA)) under oxygen plasma.Additionally, the z-axis limit switch was adjusted to accommodate the thickness of the glass slide on the build plate.Our method significantly improves transparency and resolution, enabling precise segmentation of microscale objects from analyzed images obtained through a microscope.The enhanced optical clarity and microchannel resolution of the device can facilitate imaging and characterization of microparticles, thus paving the way for high-throughput cell sorting applications.This interdisciplinary approach integrates knowledge from core Biomedical Engineering courses, emphasizing applications in cell and bioimaging.
Vargas et al. (Tue,) studied this question.