Supplementary Figure 12 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis

(A) SiRNA-mediated knockdown of KDM4A reflected the phenotype seen with QC6352 treatment in WiT49 and HEK293 cells by increasing the proportion of cells in the S-phase. Of note, SiRNA-mediated knockdown of KDM4A (but not KDM4B or KDM4C) induced aneuploidy in WiT49 and HEK293 cells (green and yellow peaks).( B) Comet assay (quantitation shown in manuscript Figure 5) demonstrated DNA damage (tails shown with white arrows) in WiT49 and HEK293 cells in response to siKDM4A. Doxorubicin (10nM) is shown as a positive control.