Supplementary Figure 6 from Tumor Burden Dictates the Neoantigen Features Required to Generate an Effective Cancer Vaccine

Phenotype characterization of total CD8+ and nAg #23+ CD8+T cells in presence and absence of the “Help”. A) Schematic representation of the experimental set up. B) Representative FACS gating strategy for intratumoral CD8+ T cells. C-D) Phenotype characterization of CD8+ T cells infiltrating the tumors (C, n=14-17) and the tumor draining lymph nodes (D, dLN, n=5-7). The frequency of Exhausted, Stem-like and Better effector cells is shown in Help (blue bars) and No Help (red bars) groups. E) Representative FACS gating strategy for intratumoral nAg #23 specific CD8+ T cells. F-G) Phenotype characterization of intratumoral nAg #23 specific CD8+ T cells collected from mice immunized with Help and No Help vaccines at day 9 post vaccination (n=7-12). The frequency of #23+ specific CD8+ T cells (F) and Exhausted and Transitory (G) is shown in Help (blue bars) and No Help (red bars) groups. The phenotypic markers used to analyze each subtype are reported in the Material and Methods section. Results are from two independent experiments. Two-Way Anova with multiple comparison analysis or Mann Whitney test were performed between Help and No Help groups. *** p=0.0005; * p<0.05; ns = not significant.