Supplementary Figure 7 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis

QC6352 treatment (25nM x 72 hours) reduces nucleoli in WiT49 and HEK293. (A) KDM4A (red) and the nucleolar marker fibrillarin (green) co-localize in the nucleoli [also represented by areas of reduced DAPI staining (blue) in the nucleus due to incompact chromatin in nucleoli] in WiT49 cells. (B) QC6352 treatment caused a reduction in the fibrillarin/nuclear area (determined by the area of fibrillarin/area DAPI) in WiT49 cells (p<0.0001), representing a reduction of nucleoli. (C) Similar findings were found in QC6352-treated HEK293 cells, which also exhibited a (D) reduction of the fibrillarin/nuclear area (p<0.0001).