Oxford Nanopore Technologies (ONT) direct RNA sequencing v1

Q: What type of study is this?

This is a Experimental Study study.

Anita Adami; Raquel Garza

doi:10.17504/protocols.io.36wgqpeo3vk5/v1

October 3, 2025Open Access

Oxford Nanopore Technologies (ONT) direct RNA sequencing v1

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Key Points

This protocol covers the entire workflow of ONT direct RNA sequencing, ensuring reliable results.
Key steps include RNA extraction, mRNA poly(A) selection, and library preparation for sequencing.
Direct RNA sequencing offers advantages in capturing full-length transcripts and complex RNA structures.
Efficient data analysis methods are crucial for interpreting results from ONT direct RNA sequencing.

Implication

Step-by-step protocol outlines RNA extraction and data analysis for RNA sequencing using ONT.

Abstract

Step-by-step protocol describing the workflow for ONT direct RNA sequencing including: - RNA extraction - mRNA poly(A) selection - library preparation for ONT direct RNA sequencing - ONT direct RNA data analysis

Discussion

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Cite This Study

Adami et al. (2025) studied this question.

synapsesocial.com/papers/68e70db290569dd607ee61f3 https://doi.org/10.17504/protocols.io.36wgqpeo3vk5/v1

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Oxford Nanopore Technologies (ONT) direct RNA sequencing v1

Key Points

Implication

Abstract

Discussion

Cite This Study

Oxford Nanopore Technologies (ONT) direct RNA sequencing v1

Key Points

Implication

Abstract

Discussion

Cite This Study

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