The examining on DNA binding activities of tetrakis-(4-tritylphenoxy)-phthalocyanine copper (II) phthalocyanine complex

Abstract Cu (II) phthalocyanine complex had been previously synthesized by reacting 4-(4-tritylphenoxy)phthalonitrile compound. The structure of obtained CuPc complex had been analyzed by absorption titration, infrared and NMR spectroscopies techniques. The DNA interacting property for the complex was analyzed in various concentration of CT-DNA utilizing elctronic absorption, emission spectroscopy, gel agarose electrophoresis and thermal melting procedures. In this report, the binding constant value for the complex was also estimated. Absorption and fluorescence spectroscopic processes proved that CuPc interacts by CT-DNA. At the the same time, the thermal melting and electrophoresis technics were practiced to analyze the intercating property of CuPc by DNA. The thermal melting and electrophoresis assays supported that CuPc combines with DNA via an intercalative interacting mechanism. The obtained findings demonstrated that the complex connects to DNA by an intercalative interacting mode. Therefore, CuPc complex may have potential cancer therapeutic agent.