First Report of Tomato Yellow Mottle-Associated Virus Infecting Paris polyphylla var. yunnanensis in Yunnan, China

HomePlant DiseaseAhead of PrintFirst Report of Tomato Yellow Mottle-Associated Virus Infecting Paris polyphylla var. yunnanensis in Yunnan, China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst Report of Tomato Yellow Mottle-Associated Virus Infecting Paris polyphylla var. yunnanensis in Yunnan, ChinaShaomei Zi, Like Gao, Rex Frimpong Anane, Yan Li, Kai Yan, Xiyan Zhao, Hong Cai, and Mingfu ZhaoShaomei ZiKey Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaResearch and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China, Like GaoKey Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaResearch and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China, Rex Frimpong AnaneMonash Biomedicine Discovery Institute, and Department of Biochemistry and Molecular Biology, Monash University, Clayton VIC 3800, Australia, Yan LiKey Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaPu'er Institute of Pu-erh Tea, Pu'er 665000, China, Kai YanKey Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaResearch and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China, Xiyan ZhaoKey Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaResearch and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China, Hong Cai†Corresponding authors: M. F. Zhao; E-mail Address: [email protected], and H. Cai; E-mail Address: [email protected]Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, China, and Mingfu Zhao†Corresponding authors: M. F. Zhao; E-mail Address: [email protected], and H. Cai; E-mail Address: [email protected]https://orcid.org/0000-0002-1186-7203Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaResearch and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaAffiliationsAuthors and Affiliations Shaomei Zi1 2 Like Gao1 2 Rex Frimpong Anane3 Yan Li1 4 Kai Yan1 2 Xiyan Zhao1 2 Hong Cai1 † Mingfu Zhao1 2 † 1Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, Kunming 650201, Yunnan, China 2Research and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China 3Monash Biomedicine Discovery Institute, and Department of Biochemistry and Molecular Biology, Monash University, Clayton VIC 3800, Australia 4Pu'er Institute of Pu-erh Tea, Pu'er 665000, China Published Online:8 Apr 2024https://doi.org/10.1094/PDIS-10-23-2084-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleTomato yellow mottle-associated virus (TYMaV) is a negative-sense single-stranded RNA virus belonging to the genus Cytorhabdovirus in the family Rhabdoviridae. The virus was first reported to infect tomato (Solanum lycopersicum L.) in China (Xu et al. 2017). Recently, TYMaV has been reported to infect Solanum nigrum (Z. Li et al. 2023), pepper (Capsicum annuum L.) (C. Li et al. 2023), and Nicotiana benthamiana (Zhou et al. 2019). Paris polyphylla var. yunnanensis is a perennial herb in the family Melanthiaceae and is of high medicinal and economic importance. At present, viruses reported to infect P. polyphylla var. yunnanensis include Paris mitovirus 1 (Chen et al. 2022), Yunnan Paris negative-stranded virus (L. Chen et al. 2021), and Paris virus 2 (Z. Chen et al. 2021). In 2022, diseased leaves of P. polyphylla var. yunnanensis showing yellowing and chlorotic spots were found in the area of Pu'er, Yunnan, China. To identify the causal pathogens, symptomatic diseased leaf samples from a single plant were collected and total RNA was extracted using the Plant RNA Kit (Omega Biotek, Norcross, GA, U.S.A.). The extracted RNA was sequenced by high-throughput sequencing on an Illumina HiSeq 2500 platform (OE Biotech, Shanghai, China), and 26,036,142 raw reads were obtained. The adaptor and the low-quality reads were removed by Trimmomatic software to obtain 24,976,514 clean reads, which were assembled into 90,624 contigs using the de novo assembly tool Trinity. BLAST analysis of the 111 virus-like contigs obtained from the transcript assembly showed that 89 virus contigs were associated with TYMaV, while 22 contigs were related to Paris alphapartitivirus 1. One virus contig consisting of 13,403 nucleotides (nt) shared high sequence similarity to the genome sequence of TYMaV (98.65% identity, ON314142.1). To confirm this result, sequence-specific primers 206F (5′-AGCAATGGCACGCAGGTC-3′) and 915R (5′-CTTCCCACCATTAGAGCC-3′) were designed based on the sequence of the assembled viral contig and used to amplify the viral sequences (that had high sequence similarity to TYMaV) from five samples (collected Pu'er) suspected of viral disease infection by using reverse transcription polymerase chain reaction (RT-PCR). The PCR products were cloned into the pGEM-T vector (Promega Corporation, Fitchburg, WI, U.S.A.), and the 5′- and 3′-terminals of the viral genome were amplified using 5′ RACE and 3′ RACE kits (Sangon Biotech, Shanghai, China). The amplified fragments were sequenced using the Sanger sequencing method (Sangon Biotech, Shanghai, China) to obtain the complete genome sequence of TYMaV. The obtained complete genome sequence was consistent in length with the de novo assembly sequence (OR910018), with some base differences and a nucleotide sequence homology of 99.82%. The full-length genome of the TYMaV isolate from P. polyphylla var. yunnanensis (TYMaV-PE, OQ615773) had 13,403 nucleotides, with 98.38% nucleotide sequence identity to the reference genome (OM827246.1) and 97.04 to 99.45% amino acid sequence similarity to the seven proteins N, P, P3, M, G, P6, and L of the TYMaV reference genome (Peng et al. 2022.). Five leaf samples suspected of viral disease from Pu'er were collected and further analyzed by indirect ELISA with TYMaV antiserum (Z. Li et al. 2023), while using leaves of healthy P. polyphylla var. yunnanensis as a negative control. The results showed positive reactions for all five diseased samples, consistent with the RT-PCR test results. Taken together, these results indicate that P. polyphylla var. yunnanensis can be naturally infected by TYMaV. To the best of our knowledge, this is the first report of TYMaV infecting P. polyphylla var. yunnanensis. This study will serve as an important reference for the study of the host range of TYMaV. Further studies are needed to understand the mode of transmission of TYMaV between P. polyphylla var. yunnanensis and other host plants and its pathogenicity and the economic significance in P. polyphylla var. yunnanensis.The author(s) declare no conflict of interest.References:Chen, L., et al. 2021. Arch. Virol. 166:3199. https://doi.org/10.1007/s00705-021-05191-yCrossref, ISI, Google ScholarChen, Z., et al. 2021. Arch. Virol. 166:2045. https://doi.org/10.1007/s00705-021-05087-xCrossref, ISI, Google ScholarChen, Z., et al. 2022. Arch. Virol. 167:645. https://doi.org/10.1007/s00705-021-05339-wCrossref, ISI, Google ScholarLi, C., et al. 2023. Plant Dis. 107:1640. https://doi.org/10.1094/PDIS-09-22-2050-PDNLink, Google ScholarLi, Z., et al. 2023. Plant Dis. 107:592. https://doi.org/10.1094/PDIS-05-22-1096-PDNLink, ISI, Google ScholarPeng, M., et al. 2022. Microbiol. Resour. Announc. 11:e00297-22. https://doi.org/10.1128/mra.00297-22Crossref, Google ScholarXu, C., et al. 2017. J. Virol. 91:001733‐17. 10.1128/jvi.00173-17Google ScholarZhou, X., et al. 2019. J. Virol. 93:002966‐19. 10.1128/jvi.00296-19Google ScholarFunding: This project was made possible through the support of the National Key R&D Program of China (2021YFD1000204).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Just PublishedSubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Published: 8 Apr 2024Accepted: 26 Feb 2024 Information© 2024 The American Phytopathological SocietyFundingNational Key R&D Program of ChinaGrant/Award Number: 2021YFD1000204KeywordsChinaCytorhabdovirusParis polyphylla var. yunnanensistomato yellow mottle-associated virusThe author(s) declare no conflict of interest.PDF download