L umpy skin disease (LSDV) is of significant economic impact for the cattle industry in Africa, Middle East and Egypt.The disease is spreading aggressively posing threats to cattle industry.Due to crossprotection, Neethling and sheep pox virus (SPV) vaccines have been used for cattle against LSDV.In this work, living attenuated Neethling and sheep pox vaccines were used for vaccination of cattle and evaluated under field conditions.14susceptiblecalves were vaccinated with live attenuated Neethling vaccine and4 cattle were vaccinated with live attenuated sheep pox vaccine with 8cattleas non-vaccinated control (4cattle for each).The humeral immunity was measured by double antigen ELISA while, cellular immune response were evaluated by interferon gamma (IFN-γ) concentration real time PCR.Humeral immune response of cattle vaccinated with SPV vaccine evaluated by ELISA, showed that the immune response begins at 7 th dayPV with absorbance of 1.4 ±0.075 then by the 14 th & 28 th DPV with mean ELI-SA 2.02 ±0.0015 and 2.5 ±0.085 then at 7 th week with a mean of 2.9 ±0.1755 then declined to 1.6 ±0.957 at 4m while, IFN-γ was expressed at 2 DPV with a fold change 1.75-3.31and at 7 DPV with fold change 1.22-1.74.ELISA of Neethling vaccinated group showed response at 30 th DPV with absorbance of 1.69 ±.07815 then by 40 th DPV with titer of 1.96 ±.09141 then at 7weeks with a mean 2.45 ±.055 then1.82±.1066at4m PV, Then IFN-γ wasexpressedat2 days PV withafoldchange6.52-11.16andat7th DPV withfoldchange1.22-1.78.According to the current study results, humeral and cellular immune response of two living attenuated Capripox viruses' vaccines were evaluated at the field conditions by ELISA and IFN-γ expression that proved to be reliable and sensitive techniques for assessment of vaccine potency.
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Elsayed et al. (2024) studied this question.
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