Comparison of Antimicrobial Resistance in Escherichia coli Strains Isolated from Diseased Broiler and Cattle based on Phenotypic and Genotypic Analysis

E scherichia coli infections in poultry and animals have become a global problem.Currently, little is known regarding the epidemiology of extended-spectrum beta-lactamases (ESBLs) producing Escherichia coli in infected poultry and cattle.This study aimed to identify antibiotic resistance patterns and ESBL-producing genes in E. coli strains recovered from diseased poultry and animals in northern Egypt.A total of 100 consecutive, no duplicate clinical samples from diseased broiler poultry and 50 samples from diseased cattle were collected during a one-year period.ESBL was found in 44% (24 out of 54) isolates of E. coli isolated from infected poultry positive for double disc diffusion, and 66% (16 out of 24) isolates of E. coli recovered from cattle positive for double disc diffusion.ESBL genes blaSHV, blaTEM, blaVEB, and blaGES) were detected in strains by multiplex polymerase chain reaction with gene -specific primers.From 40 phenotypically positive ESBL isolates, we discovered that blush, blaTEM, blactx-m1, and blactx-m9 (100%) were the most prevalent genotypes in cattle isolates, whereas blaGes and blactx-m9 (70% of isolates from poultry).This is the first study to look at ESBL resistance patterns and ESBL-producing genes in E. coli isolated from poultry and cattle.Our analysis found varying occurrences among isolates from various hosts.