Protocols for entomotoxicological developmental study of Phormia regina (Megnin) v1

This protocol was developed specifically for examining the impact of drug exposure on forensically important phenotypes (i.e., developmental duration, size, survivorship) of larval blow flies. This protocol was implemented using a laboratory colony of the black blow fly, Phormia regina (Meigen), a common and abundant blow fly in the eastern and southeastern United States. The end goal of this protocol is the generation of drug-specific developmental datasets that can be used for estimating larval age, which can be used in death investigations to then infer a minimum postmortem interval.