Synthesis, Characterization and Study Biological Activity of Substituted 4-Amino -3,5-Bis (2,4-dichloro phenoxy)-1,2,4-Triazole

In this paper, the ester (2) was prepared by reacting 2,4-dichlorophenoxy acetic acid with methyl alcohol and using concentrated sulfuric acid as a catalyst in this reaction, which, when reacted with hydrazine hydrate, obtained acid hydrazide.(3), under reflux conditions with dimethyl sulfoxide and at solvent temperature, the compound 4-amino-3,5-bis-(2,4dichlorophenoxy)-1,2,4-triazole was obtained (4).It was obtained and used to prepare a series of new Schiff bases (5a-e) through their reaction with different substitutes of benzaldehyde.The completeness and purity of all the prepared compounds were confirmed by thin-layer chromatography (TLC) and showed some physical and spectroscopic properties, namely (FT -IR 1H-NMR and 13C-NMR).In addition, the biological activity of the prepared compounds was studied, and their effectiveness against different types of bacteria such as Bacillus, Escherichia coli, and Staphylococcus aureus was discussed.Studies of mainly fungi and bacteria have shown good efficacy against the mentioned species using disc diffusion technology.