GC-MS comparative chemical composition of essential oils and volatile compounds of eryngium planum l. using classical hydrodistillation, ultrasound-assisted hydrodistillation and headspace solid-phase microextraction. Antimicrobial activity

This study presents the extraction of essential oils and volatiles of Eryngium planum (E.planum) by classical hydrodistillation (HD), ultrasoundassisted hydrodistillation (UAHD) and headspace solid-phase microextraction (HS-SPME).The GC-MS analysis showed that the essential oil of E. planum obtained by UAHD contains the following majority compounds: β-copaene (11.97%), cis-chrysanthenyl acetate (10.14%), (E)-β-farnesene (6.79%), γgurjunene (6.53%), caryophyllene (5.73%), germacrene B (3.93%), (+)-cisverbenol,2-methylpropionate (2.87%), β-selinene (2.73%).The GC-MS analysis showed that E. planum volatiles extracted by HS-SPME contains as majority compounds: cis-chrysanthenyl acetate (30.39%), (E)-β-farnesene (11.71%), γ-maaliene (7.69%), β-elemene (7.26%), caryophyllene (6.5%), βselinene (4.72%), δ-cadinene (4.72%), β-copaene (4.61%), α-pinene (4.51%), γ-muurolene (2.98%).By using the UAHD method, the yield of E. planum oil was increased by 27.27% compared with the classical HD method.The essential oil of E. planum showed an excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus.