Histopathological Effects of Nicotine on Rats Pulmonary Cell Treated with Zinc and Vitamin D

The present study sought to determine the role of vitamin D and zinc in protecting against nicotine stress by altering the alveolar response and modulating antioxidant mechanisms. Thirty mature adults Wistar rats, used as a model for mammals, were maintained at 23 ± 2 C°, randomly divided into five equal groups, and then given therapy for 14 days. G1: Nicotine was administered at a dose of 0.0015g/kg b.w. I.P., G2: zinc was administered orally at 0.06 g/kg b.w., G3: Orally administration vitamin D at 250 µg/kg b.w., and G4: both zinc and vitamin D were administered orally at the same dose as nicotine, 0.0015g/kg b.w. I.P. Rats were anesthetized with ketamine at the end of the treatment period. All groups of rats had lung samples extracted rapidly; alveolar groups of samples were cleaned and placed in physiological used 10% of neutral buffered formalin for tissue fixation. Then lung suction were repaired for histopathological study. The results showed highly significant protective effects of zinc and vitamin D in the tissues of the lung in G4. Zinc and vitamin D group G4 decreased the effectiveness of nicotine when compared with the G1 nicotine group. Protective effect of zinc and vitamin D by decreased MNCS infiltration with thickening and slightly congested pulmonary blood vessels without emphysema and pulmonary edema