Purification of viral RNA from cell culture isolates or FTA cards using MagMAX Viral RNA Isolation Kit v1

This protocol describes the viral nucleic acid recovery and purification from poliovirus isolates either in their original liquid form or spotted onto FTA cards. The method utilises the magnetic bead based MagMAX ‱ Viral RNA Isolation Kit. When using isolates directly, progress to one of the extraction workflows straight after reagent preparation, skipping Section 2. To process FTA card discs follow the procedures in Sections 1 and 2 in full before extraction. To perform manual extractions follow Workflow A, for automated extraction on King Fisher Duo Prime follow steps in Workflow B, and for automated extraction on King Fisher Flex follow Workflow C.