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Desalting of proteolytic peptides from conditioned media elution using C18 Hydrophilic–Lipophilic Balance (HLB) Cartridges in preparation for downstream proteomic profiling. For desalting of proteolytic peptides from conditioned media Step 4 is modified as we typically reconstitute in an appropriate volume of 0.2% FA rather than by weight/volume due to the input material for digestion also being based on a proportion of the volume of concentrated conditioned media.
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Bons et al. (2024) studied this question.
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