Antimicrobial activity and identification of purified bacteria obtained from soil

The soil surrounding the plant root where root exudate migrates, and microbiological activity is exceptionally high is called the rhizosphere.This soil bacteria associated with the rhizosphere known as plant-growthpromoting rhizobacteria (PGPR) produce great antibiotics with many applications in industries, drugs, and medicines development etc. Numerous studies have demonstrated the antimicrobial activity of PGPR against bacterial and fungal pathogens.Due to the extensive use of antibiotics, drug-resistant microbe infections are increasing globally.There is an urgent need for an alternative therapeutic approach.Bacteria from the rhizosphere and their secondary metabolites can be a promising source of novel antimicrobial compounds.The goal of this project is to isolate bacteria, especially focus on actinobacteria from the rhizosphere of Ocimum tenuiforum and Tinospora cordifolia and to evaluate their potential for growth inhibition against pathogens by dual-culture plate assay.A total of twenty-seven bacteria showing clear zones were isolated and gram staining was performed.All of them were gram negative and rod-shaped except R13 which was gram positive.