Overexpression of mitofusin-2 (MFN2), a mitochondrial fusion protein, is frequently associated with poor prognosis in cervical cancer patients.Here, I aimed to investigate the involvement of MFN2 in cervical cancer progression and determine the effect of MFN2 on prognosis in cervical cancer patients.After generating MFN2-knockdown SiHa cells derived from squamous cell carcinoma, I investigated the effect of MFN2 on SiHa cell proliferation using the Cell Counting Kit-8 assay and determined the mRNA levels of proliferation markers.Colony-forming ability and tumorigenesis were evaluated using a colonyformation assay and tumor xenograft mouse models.The migratory and invasive abilities associated with MFN2 were measured using wound-healing and invasion assays.Wnt/β-cateninmediated epithelial-mesenchymal transition (EMT) markers related to MFN2 were assessed through quantitative RT-PCR. MFN2-knockdownSiHa cells exhibited reduced proliferation, colony formation, migration, invasion, and tumor formation in vivo.The motility of SiHa cells with MFN2 knockdown was reduced through Wnt/β-catenin-mediated EMT inhibition.MFN2 promoted cancer progression and tumorigenesis in SiHa cells.Overall, MFN2 could serve as a therapeutic target and a novel biomarker for cervical cancer.BMB Rep. www.bmbreports.org
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Sung Yong Ahn (2024) studied this question.
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