Nanopore Sequencing of poliovirus isolates v1

This protocol is adapted from "Direct Detection of poliovirus and Nanopore Sequencing (DDNS) - Stool" to make it suitable for use with cell culture isolates in either a liquid form or that have been transported to the lab via FTA card. The protocol aims to amplify the VP1 region of poliovirus through a single PCR using the Q8/Y7 primer set. We use barcoded primers as this greatly simplifies the subsequent library preparation process. Primer sequences for the barcoded Q8/Y7 primers are found in Dataset S1 of Shaw et al (2020). This protocol is for use with Oxford Nanopore v14 chemistry sequencing reagents and can be used with the MinION Mk1B or GridION sequencer. Within the protocol steps, quality control checks are included and follow the workflow set out in the document "Quality Control and Data Recording for DDNS".