Why the study?
Standard cell culture poliovirus surveillance takes 2 to 3 weeks, while direct PCR detection has faced challenges with poor sensitivity and sequencing mixtures of poliovirus and enterovirus.
Does a nested PCR and nanopore sequencing protocol provide rapid and sensitive detection of poliovirus in stool and environmental samples compared to cell culture?
Population
155 stool samples in Pakistan, plus artificial mixtures and environmental samples
Comparison
Nested PCR and nanopore sequencing protocol vs cell culture algorithm
Design
Diagnostic validation study
Authors
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May enable faster poliovirus surveillance; leaves open prospective validation before routine adoption.
Does a nested PCR and nanopore sequencing protocol provide rapid and sensitive detection of poliovirus in stool and environmental samples compared to cell culture?
A novel nested PCR and nanopore sequencing protocol offers a rapid (<3 days) and highly accurate alternative to traditional cell culture for poliovirus surveillance.
Shaw et al. (2020) studied this question.
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