Key result
Direct molecular detection and nanopore sequencing is estimated to reduce pre-response VDPV2 cases by ~28%.
Why the study?
Detection of poliovirus outbreaks relies on a complex laboratory algorithm that delays confirmation and response, impacting outbreak size and eradication efforts.
Does direct molecular detection and nanopore sequencing (DDNS) reduce the time to detect poliovirus outbreaks compared to the standard laboratory algorithm in children with acute flaccid paralysis?
Population
525 stool samples from children with acute flaccid paralysis in Africa (May 2016-February 2020)
Comparison
Direct molecular detection and nanopore sequencing vs current cell-culture, PCR, and sequencing algorithm
Design
Observational analysis of laboratory data
Authors
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May enable faster VDPV2 detection in surveillance; leaves open prospective validation of modeled case reductions.
Observational (n=525)
Yes
Does direct molecular detection and nanopore sequencing (DDNS) reduce the time to detect poliovirus outbreaks compared to the standard laboratory algorithm in children with acute flaccid paralysis?
Effect estimate: 28% reduction (95% CI 12-42)
p-value: p=<0.001
Direct molecular detection and nanopore sequencing could significantly accelerate poliovirus outbreak response and reduce outbreak size.
Shaw et al. (2021) conducted an observational in Poliovirus outbreaks (VDPV2) (n=525). Direct molecular detection and nanopore sequencing (DDNS) vs. Standard laboratory algorithm (cell-culture, PCR, and sequencing) was evaluated on Number of VDPV2 cases before a response (28% reduction, 95% CI 12-42, p=<0.001). Direct molecular detection and nanopore sequencing could reduce the number of VDPV2 cases before an outbreak response by an estimated 28% (95% CrI 12-42%).
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