Key result
Real-time PCR (singleplex and multiplex) detected poliovirus in 76.25% and 75% of samples compared to 68.75% by culture, though the difference was not statistically significant.
Why the study?
Does real-time PCR improve the detection of poliovirus in stool samples of recently vaccinated children compared to culture?
Population
80 stool samples from recently vaccinated Indian children
Comparison
Single and one-step multiplex real-time PCR assays vs Culture (gold standard)
Design
Cross-sectional
Authors
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May accelerate poliovirus surveillance; leaves open prospective validation before supplanting culture as gold standard.
Cross-Sectional (n=80)
Does real-time PCR improve the detection of poliovirus in stool samples of recently vaccinated children compared to culture?
Absolute Event Rate: 76.25% vs 68.75%
p-value: p=not statistically significant
Real-time PCR (singleplex and multiplex) is a faster and potentially more sensitive alternative to culture for detecting poliovirus in stool samples, although the difference in sensitivity was not statistically significant in this cohort.
Giri et al. (2017) conducted a cross-sectional in Sabin poliovirus shedding (n=80). Real-time PCR (singleplex and multiplex) vs. Culture was evaluated on Detection of poliovirus from stool samples (p=not statistically significant). Real-time PCR (singleplex and multiplex) detected poliovirus in 76.25% and 75% of samples compared to 68.75% by culture, though the difference was not statistically significant.
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