Flux balance analysis (FBA) is a mathematical approach to finding an optimal net flow of mass through a metabolic network that follows a set of instructions defined by the user. This protocol covers the mathematical principles behind FBA and provides coding examples that can be followed using Python3. A basic understanding of the following aspects of linear algebra is advantageous: vectors, matrices, transposition, matrix multiplication, and matrix decomposition.
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Carter et al. (2024) studied this question.
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