FIGURE 4 from Activity of the Ubiquitin-activating Enzyme Inhibitor TAK-243 in Adrenocortical Carcinoma Cell Lines, Patient-derived Organoids, and Murine Xenografts

TAK-243 inhibits ubiquitylation and induces UPR in ACC cell lines. A, Time course for ubiquitylated proteins and free ubiquitin in cells treated with TAK-243 (500 nmol/L, 2 or 4 hours). The indicated proteins were evaluated by Western blotting. B, Quantitation of the data shown in A. C, Concentration response of ubiquitylated proteins and free ubiquitin in cells treated with the indicated concentrations of TAK-243 for 2 hours. The indicated proteins were evaluated by Western blotting. D, Quantitation of the data shown in B. E, UPR induced by TAK-243 (500 nmol/L, 2 or 4 hours). The samples were from the same cellular lysates as in A, and GAPDH blots were intentionally reused for presentation. The indicated proteins were evaluated by Western blotting.