Experimental assessment of the state of the rat organism in response to exposure to cadmium chloride for three months and remission

Background: Heavy metals could be considered one of the factors determining the development of man and society, but their spread entails the danger of environmental pollution, which can affect the health of society itself. The aim of the study: The aim of the study was to study the effect of cadmium chloride on the liver of rats by assessing changes in biochemical parameters in blood serum and determining the expression level of Mt1a and Mt2a genes after exposure to a toxicant for three months and remission lasting one month. Materials and methods: 40 individuals of white outbred rats were selected by random sampling from the general laboratory population, taking into account the sex ratio of 1:1. Then the animals were distributed into 5 males and 5 females, respectively, into four groups. Individuals of the three experimental groups were orally administered an aqueous solution of CdCl 2 daily for three months at concentrations of 1, 10 and 100 micrograms/kg. Results: According to the results of the analysis of the expression of Mt1a and Mt2a genes in liver tissue in response to the daily intake of cadmium in these concentrations during the experiment, statistically significant differences between the study groups were revealed. The differences between the levels of AST, LDH and total protein in the blood serum of the control and experimental groups also turned out to be statistically significant. Conclusion: The study demonstrates the relationship between the dosages of cadmium obtained by experimental animals for three months with one month of remission and an increase in the expression of Mt1a and Mt2a metallothionein genes in the liver, as well as with changes in some biochemical parameters compared with the control group. The results of numerous studies in this field complement each other and form a reliable information base for the prevention and treatment of toxic poisoning with cadmium compounds.