Fluorescence quenching effects on decoherence and energy transfer dynamics of green fluorescent proteins

The study of decoherence in biological systems has become an essential area of research for quantum mechanics. Fluorescence quenching is a physiochemical process that causes the decrease in fluorescence intensity of a fluorescent sample. Changing the surrounding environment of fluorescent proteins (FPs), by adding a fluorescence quenching agent could potentially have a large impact on the dynamical processes within these biological systems. Environmental influence on protein-protein interactions is not a well understood process, when looking at energy transfer dynamics of FPs. we attempt to establish the impact on the energy transfer dynamics between fluorophores by introducing varying concentrations of external quenchers to the FPs environment. We examine the photophysical properties and spectral changes of the FPs, after adding external quenchers to both the monomeric and dimeric structures to further characterise the energy transfer dynamics.