Quantitative imaging of murine organs with deep ultraviolet fluorescence microscopy

Quantitative fluorescence imaging of entire organs at the cellular level is crucial in understanding tissue structure and function. However, it is often time-consuming and labor-intensive to section tissue for high-resolution microscopy. We developed a deep ultraviolet fluorescence microscope for rapid surface imaging of thick unsectioned tissue at cellular resolution. Fast staining and tissue microscopy protocols were designed for practical use cases. We investigated the effects of vancomycin on mouse kidneys and also conducted studies on uterine ageing and its association with extracellular matrix protein expression. Deep ultraviolet fluorescence microscopy offers a promising approach in quantitative mapping of whole organs.