Using meditope-based antibody labeling to improve fluorescence lifetime FRET imaging

Herein, we will use Macroscopy Fluorescence Lifetime Forster Resonance Energy Transfer (MFLI FRET) to quantitatively image in vivo and non-invasively the efficacy of receptor-targeted drug delivery systems. We have established an antibody-to-target imaging pipeline to develop novel near-infrared (NIR)-labeled probes to interrogate and quantify the dynamics of drug-receptor binding in HER2 breast cancer tumor xenografts.