Phenotypic analysis of root nodules based on paraffin fixation v1

The mutualistic relationship between leguminous plants and rhizobia bacteria stands out as one of the most crucial, primarily because of its capacity to convert atmospheric nitrogen (N2) into ammonia (NH3). Recent years have witnessed a surge in studies focusing on the regulation and establishment of this symbiosis, leading to the development of protocols aimed at scrutinizing the phenotype of the pivotal structures facilitating nutrient exchange between the symbiotic partners – the nodules. The subsequent protocol outlines a highly effective method for paraffin processing of nodules, enabling a comprehensive phenotypic analysis. This protocol serves as a valuable analytical tool for research endeavors delving into the intricate details of the rhizobia-legume symbiosis.