Large chromatic shift objective for chromatic confocal microscopy

The chromatic confocal microscope is an innovative method to acquire axial information simultaneously but suffers from limited imaging depth. We designed an 800um chromatic shift chromatic objective and manufactured it by precision diamond turning. By utilizing this objective, our chromatic confocal system can capture a large depth axis information simultaneously without stage scanning, while maintaining 780um lateral resolution. Biology tissues are visualized under this microscope to evaluate its performance.