Extracellular vesicles derived from mesenchymal stromal cells and red blood cells feature distinct biomolecular corona dynamics

The Biomolecular Corona (BC) is a typical phenomenon that occurs at the interface between the nanomaterial and the surrounding media, involving the adsorption or the desorption of macromolecules at this interface. Studying BC dynamic properties on soft nanomaterials (i.e., liposomes or extracellular vesicles) proves to be challenging. Advancing in situ methodologies for investigating BC on soft nanoparticles is essential for elucidating the intricate interplay between surface composition, properties, and dynamic BC formation. In this study, we present new evidence of distinct dynamics in BC formation for two extracellular vesicle (EV) subtypes. Utilizing Fluorescence Correlation Spectroscopy (FCS) coupled with a fluorescent monoclonal antibody as a corona probe enabled the characterization of macromolecule exchange at the EV surface and the dynamic evolution of the BC.