EVennCorona: Annotating Protein Corona of Extracellular Vesicles by Venn Diagram

Extracellular vesicles (EVs) circulating in human blood are coated by adsorbed plasma proteins, termed the EV protein corona. The corona is an intrinsic property of EVs in vivo, forming through selective adsorption of tissue microenvironment secretome components and plasma proteins, and modulates key EV biological functions including cellular targeting, biodistribution, immunomodulation and tissue regeneration. These properties have emerging implications for therapeutic EV bioengineering. However, the corona also confounds plasma EV biomarker discovery by obscuring intrinsic vesicular proteins. Despite its importance, systematic and accessible annotation of EV-associated protein corona is lacking. EVennCorona is a web-based resource for annotating plasma-derived EV proteins using Venn diagram-based comparisons with curated protein corona reference datasets. The database integrates experimental validated comparative proteomics of EVs with and without corona across diverse EV populations. EVennCorona facilitates prioritisation of intrinsic EV proteins for plasma EV biomarker discovery and supports investigation of EV corona biology. The resource is available at https://schuti.github.io/EVennCorona.